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. 2011 Jan 1;22(1):153–164. doi: 10.1091/mbc.E10-08-0732

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© 2011 Helmstaedt et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 5: — Yeast two-hybrid interaction of A. nidulans CandAs, CulA, and CulD. (A) Combinations of candA-C, candA-N, culA, and culD cDNAs, cloned into the bait and prey vector, and/or empty bait (b) and prey (p) control vectors (O) were viable (pos) but unable to grow under restrictive conditions (neg). Interaction resulted in leucine prototrophy or specific β-galactosidase activity (β-Gal). 10 μl of liquid cultures (OD₅₄₆ = 0.01). (B) Interaction of deneddylated CulA^K710R (CulA*) and CulD^K826R (CulD*) with CandA-N or CandA-C.