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. 2011 Jan 1;22(1):78–90. doi: 10.1091/mbc.E10-06-0504

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© 2011 Hattersley et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 8: — GFP-SENP6^C1030A localizes to the core domain of PML NBs. HeLa cells were transfected with GFP-SENP6^C1030A 36 h before immunostaining with PML antibodies. Conventional deconvolution microscopy was performed on samples and presented as projected images. Scale bar represents 10 μm (top panels). To achieve higher resolution of the indicated PML NBs, cells were analyzed by structured illumination and are images presented as individual Z-slices. SENP6 was found to localize to the core domain in 57% of cases. Scale bars represent 2 μm.