FIGURE 5:

Western blots detecting CstF-64 and CstF-64Tau. (A) Western blot revealing CstF-64 and a 70-kDa band corresponding to CstF-64Tau in total extracts of HeLa cells transfected with plasmids encoding shRNAs specific for T-cell receptor β, CstF-64, and symplekin mRNAs. CstF-64 and CstF-64Tau were detected with the C-20 antibody against the C-terminus of CstF-64. Symplekin was detected with a monoclonal anti-symplekin antibody while SmB/B′, which served as loading control, was detected with the monoclonal antibody Y12. (B) Western blot showing CstF-64Tau from the same total extracts of HeLa cells as above transfected with shRNAs specific for T-cell receptor β or CstF-64. CstF-64Tau was detected in the two right-hand lanes with a CstF-64Tau-specific rabbit polyclonal antibody, whereas in the two left-hand lanes the C-20 antibody reacting with both proteins was used.