Figure 4.

Aβ-induced paralysis in C. elegans strain CL2006 fed with nicotine. (A) Effect of nicotine at concentration 1, 10, 100 and 500 nm on Aβ-induced paralysis. (B) Effect of nicotine at concentration 1, 10, 100 and 1,000 μM on Aβ-induced paralysis. Synchronized eggs of CL2006 C. elegans were maintained at 20°C, on the 35 × 10 mm culture plates (~35 eggs/plate) containing vehicle (control) or nicotine. The treatment was given to the worm from egg stage onward until the worm completely paralyzed (35 worms in each experiment, 3 independent experiments). n.s. = not significant