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. 2011 Jan 6;6(1):e15871. doi: 10.1371/journal.pone.0015871

Figure 8. Expression of LUC-tagged core+1/ARFP construct from plasma p34 in Huh-7 cells.

Figure 8

(A) Schematic representation of the Luc-tagged constructs. Core+1/ARFP coding sequences were fused in frame with the firefly Luciferase gene, under the transcriptional control of the CMV promoter and the translational control of the HCV-1a IRES. 1: core+1/ARFP from HCV-1a H strain (pHPI8234), 2: anti-sense core+1/ARFP construct from HCV-1a H strain pHPI8235 (negative control, see arrow), 3: core+1/ARFP from patient p34 (pHPI8227).(B) Huh-7 cells were transfected with each plasmid and the relative Luciferase activity derived from each construct were determined. The LUC levels were arbitrarily set at 100% for pHPI 8234. Values plotted correspond to the mean obtained in three separate experiments carried out in duplicate. Error bars represent standard deviation. Similar results were obtained using HeLa and BHK-21 cells.