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. 2011 Jan 6;6(1):e15861. doi: 10.1371/journal.pone.0015861

Figure 6. PRO 2000 inhibits LPS signalling and inhibits the TLR4-MD2-lipid A interaction.

Figure 6

Murine embryonic fibroblast (MEF) TLR reporter cells were activated with ultra-pure LPS (A, corresponding legend in B) or with the synthetic TLR2 agonist Pam3CSK4 (B) in the presence of 10, 30 or 90 µM PRO 2000 or in medium alone. Means of triplicates ± SD are shown. For surface plasmon resonance binding assays (C–D), TLR4:MD2-complexes were immobilized on a CM5 chip (C), and binding of 100 µM PRO 2000 or low molecular weight dextran was observed, or lipid A was immobilized on a HPA chip (D) and binding of 155 nM TLR4:MD2, 1.54 µM or 77 nM PRO 2000 or combinations thereof were observed.