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ROS were determined in A: horizontally BW-resistant line H7996 and B: BW-susceptible cv. Bonny Best 48 h after infusion with 1×109 CFU/ml R. solanacearum strain UW551 or EPS I mutant UW551ΔepsB. ROS appears as a brown precipitate after leaves were stained with the in situ endogenous peroxidase-dependent histochemical stain 3,3′-diaminobenzidine (DAB). The experiment was repeated five times; representative results are shown.
