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Semi-quantitative RT-PCR analysis of the genes involved in BR biosynthesis and cell division. Total RNA isolated from whole seedlings or calli of wild type and dwf7-1 were subjected to a semi-quantitative RT-PCR assay. The tested genes are listed at left side. The number of thermo cycles for each gene ranged from 24-28. Both the UBQ10 and ACTIN2 genes were used as a loading control.
