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. 2011 Jan 6;6(1):e15868. doi: 10.1371/journal.pone.0015868

Figure 1. ATRA does not impair human T cell proliferation and augments FOXP3 expression during ex vivo expansion.

Figure 1

A. 200,000 sorted primary human Tregs were stimulated with anti-CD3 Ab loaded K.64.86 cells and cultured in the presence of 10% human serum alone for 15 days (control) or in serum-containing medium with the addition of 100 ng/ml of rapamycin (RAPA) or 10 µM ATRA. Cell counts were performed on Days 5, 8, 12 and 15 and data reflect the average of 3 independent experiments. The error bars reflect standard deviation. B. After 15 days of culture, each cell population from a single donor described in Panel A was analyzed for CD25 and FOXP3 expression by flow cytometry. C. Composite FOXP3 expression data from 6 independent experiments performed using 6 unique donors. The donor shown in Panel B is donor #4.