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| Mutation | Medusa ΔΔG (kcal/mol) ± SEM1 | Predicted Phenotype | Experimental Result |
| H3 H113A | 8.1±0.5 | Lethal | Lethal |
| H3 L126A | 4.8±0.4 | Lethal | Lethal |
| H3 A114Y | 3.0±0.4 | Lethal | Lethal |
| H3 L130A | 10.1±0.4 | Lethal | Lethal |
The standard error of mean (SEM) obtained for the Medusa calculations.
