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. 2011 Jan 6;7(1):e1001258. doi: 10.1371/journal.ppat.1001258

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This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

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A549 cells were transduced to express the indicated c-myc-tagged (A) human, (C) mouse, or (E) chicken IFITM orthologs. Two days later, cells were infected with MLV-GFP pseudotyped with the indicated viral entry glycoproteins. Pseudovirus infection was measured by flow cytometry, and normalized to that of cells transduced with vector alone. Expression of (B) human, (D) mouse, or (F) chicken IFITM protein orthologs in A549 cells assayed in (A), (C), and (E), respectively was measured by western blot using the anti-c-myc antibody (9E10). All differences in pseudovirus entry between control and IFITM-expressing cells are significant (P<0.05) except for H5(Thai) entry into murine Ifitm6- and chicken Ifitm5-expressing cells, and for H7(FPV) entry into murine Ifitm1- and Ifitm5-expressing cells. Each panel of the figure represents at least two experiments with similar results.