Fig. 2.

Lamin A/C is necessary for nuclear movement. (A) Images of LPA-stimulated Lmna^+/+ and Lmna^−/− MEFs in a wounded monolayer stained for pericentrin and β-catenin (red), nuclei (blue), and microtubules (green). Arrowheads indicate the centrosome, and the wound is located at the top. (Scale bar, 10 μm.) (B) Nucleus (red) and centrosome (blue) positions in LPA-stimulated Lmna^+/+ and Lmna^−/− MEFs. Centrosome orientation (percentage of cells) is located below the histogram. Error bars are SEM for >100 cells from at least three independent experiments. The nuclear position is significantly different between stimulated Lmna^+/+ and Lmna^−/− cells (P < 0.001). (C) Phase contrast images from movies of Lmna^+/+ and Lmna^−/− MEFs. The boxed region (Left) is shown at higher magnification (Right). Images were acquired every 5 min (time is in minutes). (Scale bar, 10 μm.) (D) Nucleus (red) and centrosome (blue) positions in NIH 3T3 fibroblasts depleted of lamin A/C and GAPDH. Centrosome orientation (percentage of cells) is shown below the histogram. Error bars are SEM for >100 cells from three independent experiments. The nuclear position is significantly different between GAPDH- and lamin A/C-depleted NIH 3T3 fibroblasts (P < 0.001).