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. 2010 Dec 20;108(1):131–136. doi: 10.1073/pnas.1000824108

Fig. 5.

Fig. 5.

Lamin A/C provides a nucleoplasmic anchor for TAN lines. (A) Panels from movies of GFP-mininesprin-2G in nuclei of LPA-stimulated Lmna+/+ (Upper) and Lmna−/− (Lower) MEFs. The wound edge is located toward the top. Images were acquired every 5 min (time is minutes after LPA stimulation). Arrows indicate TAN lines that move with (Upper) or over (Lower) the nucleus. (Scale bar, 5 μm.) (B) Traces of the movement of the front, back, and centroid of the nucleus (gray) and two TAN lines (black) in a Lmna+/+ MEF (Left) and Lmna−/− MEF (Right). (C) Velocity of the nucleus and TAN lines in the cells described in A. Error bars are SEM for 30 nuclei/TAN Lines from three experiments. Nuclear velocity in Lmna−/− MEFs is significantly different from that in Lmna+/+ MEFs (P < 0.001); there is no significant difference between TAN line velocities. (D) Panels from movies of GFP-mininesprin-2G in nuclei of LPA-stimulated NIH 3T3 fibroblasts expressing the indicated red fluorescent protein-tagged lamin A variants. The wound edge is located toward the top. Images were acquired every 5 min (time is minutes after LPA stimulation). Arrows indicate TAN lines that move with the nucleus (Upper) or over the nucleus (Lower). (Scale bar, 2 μm.) (E) Velocities of nuclei and TAN lines in the cells described in D. Error bars are SEM for 30 nuclei and TAN lines from three experiments. Nuclear velocity in E358K was significantly different from that in R482W (P < 0.001); there is no significant difference between TAN line velocities. (F) Panels from a movie of GFP-mininesprin-2G in the nucleus of LPA-stimulated NIH 3T3 fibroblasts depleted of SUN2. The wound edge is located toward the top. Images were acquired every 5 min (time is minutes after LPA stimulation). Arrows indicate a TAN line that moves over the nucleus. (Scale bar, 5 μm.) (G) Velocity of nuclear and TAN line movements in SUN2- or control (GAPDH)-depleted NIH 3T3 fibroblasts stimulated with LPA. Error bars are SEM for 17 nuclei from four experiments. Nuclear velocity in SUN2-depleted cells was significantly different from that in GAPDH-depleted cells (P < 0.05); there was no significant difference in TAN line velocities.