Table 1.

Effect on pronuclear meeting and centration of knockdown of the top nine genes identified in PhenoBank analysis as involved in centration

					Under gpr-1/2 (RNAi)‖
Rank*	Genotype	Meeting defect†	Centration defect‡	Score, %§	Meeting defect†	Centration defect‡	Score, %§
1	dyrb-1(RNAi)	0/9	9/9	100	2/13	13/13	85
2	dnc-1(RNAi)	2/7	7/7	71	8/12	12/12	33
2	dnc-2(RNAi)	2/6	6/6	67	4/9	7/9	33
3	arp-1(RNAi)	5/12	12/12	58	8/11	11/11	27
4	gpb-1(RNAi)	0/10	10/10	100	0/14	2/14	14
5	let-754(RNAi)	0/9	6/9	67	0/13	0/13	0
5	gpc-2(RNAi)	0/8	8/8	100	0/13	0/13	0
5	lrg-1(RNAi)	0/12	12/12	100	0/15	0/15	0
—	csn-1(RNAi)	0/14	4/14	29	—	—	—
—	WT¶	0/17	0/17	0	0/7	0/7	0
—	dhc-1(RNAi)	6/7	6/7	0	8/8	8/8	0

*Ranking of genes based on score [under the gpr-1/2 (RNAi) condition].

^†Number of embryos displaying pronuclear meeting defect divided by total number of embryos in each RNAi.

^‡Number of embryos displaying centration defect divided by total number of embryos in each RNAi.

^§Percentage of embryos displaying centration defect but not pronuclear meeting defect.

^‖Knockdown of top eight genes performed under the gpr-1/2 (RNAi) condition.

^¶CAL0092 strain was used as WT.