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. 2010 Dec 20;108(1):290–295. doi: 10.1073/pnas.1005758108

Fig. 3.

Fig. 3.

DSS-induced colitis histopathology is less severe in 6−/7− mice than in 6+/7− mice. (A–E) The colons of the DSS-treated and untreated 6+/7− and 6−/7− mice were harvested, fixed, and stained. (A) Severe inflammation, in particular ulceration (black arrows), loss of crypts (large black arrowheads), and increased inflammatory infiltrate can be seen in the representative colon from a DSS-treated 6+/7− mouse. The majority of the infiltrating inflammatory cells are neutrophils, as assessed at higher power by H&E staining (black arrows in B) and by chloroacetate esterase histochemistry (pink-staining cells indicated by black arrows) (C). In contrast, the histopathology of the colon of the DSS-treated 6−/7− mouse in D resembles that of the untreated 6+/7− mouse in E. (Scale bars: 50 μm.) (F) There are significant differences in the numbers of neutrophils per high-power field (mean ± SEM) in the colon segments of DSS-treated 6+/7− and 6−/7− mice. *P = 0.007.