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. 2010 Dec 20;108(1):143–148. doi: 10.1073/pnas.1010929108

Fig. 1.

Fig. 1.

ScaPCs from muscle and sWAT, but not from eWAT, can be induced to undergo brown adipogenesis. Distinct expression profiles are associated with brown adipogenic capacity. (A) Oil Red O staining of lipid-accumulating cells in both control and BMP7 pretreated cells from iBAT, MUS, sWAT, and eWAT. (B) Quantitative RT-PCR of UCP1 in differentiated ScaPCs with and without BMP7 pretreatment. Fold-changes are given for each tissue type of BMP7-treatment over control. Black bars indicate control conditions; white bars indicate BMP7-pretreatment. Data are presented as mean ± SEM (n = 3). Asterisks denote significant differences between control and BMP7-pretreated cells. *P < 0.05; **P < 0.01; ***P < 0.001. (C–H) Quantitative RT-PCR analysis of undifferentiated Sca-1+ progenitor cells isolated from iBAT, MUS, sWAT, and eWAT: (C) Zic-1, (D) PRDM16, (E) Engrailed1 (Eng1), (F) HoxC9, (G) HoxC8, and (H) dermatopontin (DPT). Data are presented as mean ± SEM (n = 3); “a” depicts statistically significant differences (P < 0.05) between iBAT and the indicated tissue; “b” depicts statistically significant (P < 0.05) differences between MUS and indicated tissue; “c” depicts statistically significant (P < 0.05) differences between sWAT and eWAT.