Fig. 4.

BMP7 induces brown adipogenesis in primary human preadipocytes. Quantitative RT-PCR analysis of (A) fatty acid binding protein 4 (FABP4, aP2) and (B) UCP1 in human primary preadipocytes derived from subcutaneous (SQ), mesenteric (Mes), and omental (OM) white fat. Cells were seeded and cultured in the presence of either vehicle (black bars), or BMP7 (3.3 nM; white bars) throughout differentiation. All data are presented as mean ± SEM (primary cells from five to seven individual subjects were used). *P < 0.05.