Fig. 3.
Expression of cell-cycle regulators in endocrine lineage cells. Pancreases were dissected from Neurog3-Timer embryos at E17.5, and fluorescent cells were sorted by FACS into the four gates shown in Fig. 1C. In parallel, NeurogeGFP/+ and Neurog3eGFP/eGFP embryos were dissected at E14.5, and GFP-positive cells (G) were separated from GFP-negative cells (N) by FACS. The sorted cell populations were analyzed by real-time RT-PCR for mRNA-encoding cell cycle-related genes, Cdkn1a (A), Cdkn1b (B), Ccnb2 (C), and Ccnd2 (D). All expression levels were normalized to glucuronidase, β (Gusb). Each data point represents the mean ± SEM of three independent experiments.