Fig. 1.

Schematic diagram of Phase-Seq work flow. Single chromosomes were sorted into wells of a 96-well plate in which single chromosome amplifications were performed. Each amplified DNA molecule from a single chromosome (e.g., Chr19) contained a specific tag (shown in red or blue) that allowed multiplex sequencing on a high-throughput sequencing platform. Multiplexed reads were assigned to haploid genomes based on the combination of single chromosome-specific tags (shown in red or blue) and haploid genome-specific SNPs (lowercase letters in italic bold type). (Inset) FACS sorting of stained single chromosomes is based on the fluorescence patterns of Hoechst and Chromomycin, which allow reliable separation of different chromosomes (Chr18 and Chr19 are marked).