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. Author manuscript; available in PMC: 2012 Mar 1.
Published in final edited form as: Chem Res Toxicol. 2010 Sep 28;23(10):1601–1607. doi: 10.1021/tx100190k

Fig. 5.

Fig. 5

Effects on cell vitality 24 hours after treatment with racemic, (R)- or (S)-HNE. Assay and flow cytometric parameters were as described in “Experimental Procedures”. Cells populations, 24 hours after treatment, were gated as live (white bars), dead (black bars) or injured (gray bars) based on their fluorescent properties. Cells treated with (S)-HNE generated a nearly 2-fold increase in cell death when compared to either (R)- or racemic HNE. Results are representative of three independent experiments. Statistical analysis was performed using Prism.