Table 1.
Genomic changes and variability of the cell lines
| Cell line | RER status | p53 status | Mode | Rearranged chromosomes | Variability of centromere number (%) |
|---|---|---|---|---|---|
| RER− | |||||
| C70 | − | ND | 127 | 14 | 29 |
| HT29 | − | Mut | 70 | 15 | 14 |
| LIM1863 | − | NF | 80 | 9 | 12 |
| SW1417 | − | NF | 70 | 22 | 13 |
| SW403 | − | Mut | 64 | 14 | 18 |
| SW480 | − | Mut | 58, 90 | 15, 16 | 16, 30 |
| SW620 | − | Mut | 48 | 15 | 10 |
| SW837 | − | Mut | 38 | 13 | 6 |
| VACO4A | − | NF | 62 | 10 | 6 |
| Typical RER+ | |||||
| DLD1 | + | Mut | 46 | 3 | 2 |
| GP2d | + | ND | 46 | 4 | 1 |
| HCT116 | + | Mut | 45 | 3 | 2 |
| LoVo | + | NF | 49 | 3 | 2 |
| LS174T | + | NF | 47 | 0 | 2 |
| VACO5 | + | Mut | 46 | 2 | 3 |
| Atypical RER+ | |||||
| HCA7 | + | Mut | 43 | 21 | 4 |
| LS411 | + | NF | 73 | 21 | 12 |
RER status is from ref. 4. p53 status: Mut, mutation detected; NF, no mutation found; ND, not done. Mutation screening was by chemical mismatch cleavage analysis of exons 3–9 and 11 (27). The variability of centromere numbers [adapted from Lengauer et al. (19)] was obtained by counting in each of 8–15 metaphases the number of copies of each centromere, whether in normal or rearranged copies of a chromosome; noting the percentage of metaphases that have deviations from the modal centromere number; and averaging over all centromeres. The two values for SW480 are for its two major clones. Our measurements of metaphase heterogeneity were slightly lower than Lengauer et al.'s (19), presumably because we examined cells in metaphase, whereas they studied interphase nuclei, and some of the cells they observed with altered chromosome numbers may not divide.