Fig. 2.

Activation of the canonical WNT pathway by HNE. ARPE19 cells were incubated with HNE for 6 h. a Levels of phosphorylated (p)LRP6 and total LRP6 were determined by western blot analysis using 50 μg of total proteins from each sample. b Quantification of pLRP6 and (c) total LRP6 by densitometry from three independent experiments, normalised to β-actin. d Levels of phosphorylated (p)GSK3β (Ser9) and (f) cytosolic β-catenin were determined by western blot analysis as above (a). e Quantification of pGSK3β (Ser9) and (g) β-catenin levels by densitometry from three independent experiments, normalised to β-actin or glyceraldehyde-3-phosphate dehydrogenase (GAPDH) levels. Values (b, c, e, g) are expressed as per cent of control cells, given as mean ± SD, n = 3; *p < 0.05 and **p < 0.01