Habitat variables tested and their associated sample size, mean, and standard deviation. Water chemistry data were obtained from a dataset compiled by stickleback researchers beginning in 1989 (Bell & Ortí, 1994; Bell et al., 1993; Jones et al., 2003; Walker, 1997) and from the Alaska Lakes Assessment conducted by the U.S. Environmental Protection Agency in 2008. Physical habitat data were obtained from Alaska Department of Fish and Game lake files. See Willacker (2009) for details on the compilation of the dataset.