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Organization of the olvas gene and structure of the olvas-GFP vector. The upper line indicates the scale that is proportional to the organization of the medaka vasa (olvas) gene shown below. Open boxes denote the exons of the olvas gene. Olvas-GFP is composed of an enhanced GFP gene (EGFP, CLONTECH) flanked by the 5′ region (5.1 kb) including exon 1 and exon 2 and the 3′ region (0.64 kb) of olvas gene. E, EcoRI; B, BamHI; P, PstI.
