Fig. 1.

Morphologic observation of hESCs during differentiation into MSC-like cells

(a) hESCs were cultured on mitomycin-C inactivated MEFs; (b, c) Undifferentiated hESCs stained positively for SSEA-4 and Nanog, respectively; (d) hESCs treated with 10 μmol/L Y-27632 were plated into gelatin-coated dishes at low density in DMEM supplemented with 10% FBS; (e) On Day 4, non-adherent cells and debris were removed, and the adherent cells were cultured continuously in complete medium; (f) After 10 d, the cells showed a homogenous fibroblastic morphology that resembled adult MSCs