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. 2010 Oct 19;19(1):93–102. doi: 10.1038/mt.2010.214

Figure 5.

Figure 5

Nucleolin associates with glucocorticoid receptor (GCR). (a) HeLa cells stably transfected with vector or nucleolin-FLAG (NCLF) were IPed for FLAG, and stained with Coomassie blue followed by silver stain. Bands appearing in the NCLF lane but not the vector lane were cut out, processed for LC–MS/MS analysis. MS ID of each band is labeled on the right side. (b) The spectrum of a peptide from GCR identified by MS/MS analysis. Identified b and y ions are labeled bold italic. (c) HeLa cells stably transfected with vector (V) or nucleolin-FLAG expressing vector (NCLF) were immunoprecipitated with mouse monoclonal antibodies against fibrillarin (Control IP) or FLAG (FLAG IP), and immunoblotted for GCR, FLAG, and dynein-intermediated chain (DIC). (d) HeLa and 16HBEo− cells were treated with cortisone for 0–60 minutes, immunoprecipitated for GCR, and immunoblotted for nucleolin. Total nucleolin and GCR were blotted as loading controls. (e) Nucleolin bands in the GCR IP blots in d were quantified, and plotted against time.