Figure 7.

Total nucleolin expression in wild-type and cystic fibrosis (CF) mice lungs. Lung sections from (a,c,e) wild-type and (b,d,f) δF508 C57Bl6 mice were stained with rabbit antinucleolin H-250 antibody, counterstained with hematoxylin, and examined at ×200 magnification. (a,b) Triton-permeabilized sections stained without primary antibody. (c,d) Adjacent nonpermeabilized sections stained with primary antibody. (e,f) Adjacent permeabilized sections stained with primary antibody. Arrowheads in e and f represent high expression; asterisks in e and f represent low expression. BM, basement membrane; En, endothelium.