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. 2010 Oct 12;19(1):133–139. doi: 10.1038/mt.2010.218

Figure 1.

Figure 1

Lentiviral vectors to evaluate insulator functions in erythroid cells. (a) We constructed reverse-oriented GFP (rMpGFP) expression cassette under the control of an MSCV-LTR promoter or (b) β-globin (BGpGFP) expression cassette in which the globin gene was changed to GFP cDNA. A full-length HS4 insulator (1.2 kb HS4), a tandem HS4 core insulator (2 × 250b HS4), or a single core insulator (250b HS4) were inserted into the LTR, in forward or reverse orientations. GFP, green fluorescent protein; HS4, hypersensitivity site 4; LCR, locus control region; MSCVp, murine stem cell virus-long-term repeat promoter; Poly A, polyadenylation signal; UTR, untranslated region; βGp, β-globin promoter.