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. 2010 Oct 12;19(1):133–139. doi: 10.1038/mt.2010.218

Figure 6.

Figure 6

Titration of BGpGFP vectors in human erythroid culture. GFP expression of GPA+ cells was evaluated with escalating MOI of BGpGFP vectors on day 13 of human erythroid culture. The insulated vector (250b HS4 R) showed lower %GFP in MOI 10, 25, and 50 (N = 3, P < 0.05 in t-test) and lower overall GFP expression (%GFP × MFI) at MOI 25 and 50 (N = 3, P < 0.05 in t-test), compared to the uninsulated BGpGFP vector, demonstrating that the inclusion of HS4 insulator elements decreases GFP expression, and could not be overcome by increasing MOI. GFP, green fluorescent protein; HS4, hypersensitivity site 4; MFI, mean fluorescence intensity; MOI, multiplicity of infection.