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Increase in [Ca2+]i in cells incubated with PIP2:histone. (A) Bright field images of RBLs before and after incubation with 2 μM PIP2:histone for 10 min. (B) Fluorescence images of Fluo-3-loaded RBL cells incubated with 2 μM PIP2:histone for varying times. (C) Bright field images of RBL cells before and after incubation with 0.5 μM PIP2:histone for 10 min. (D) Fluorescence images of Fluo-3-loaded RBL cells incubated with 0.5 μM PIP2:histone for varying times.
