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. Author manuscript; available in PMC: 2011 Dec 1.
Published in final edited form as: Mol Microbiol. 2010 Oct 26;78(6):1510–1522. doi: 10.1111/j.1365-2958.2010.07420.x

Fig. 1.

Fig. 1

Physical and transcriptional organization of the cdhR region in the P. gingivalis 33277 chromosome. (A) Genetic organization of the cdhR region. Arrows indicate the direction of transcription. (B) RT-PCR of genomic or cDNA with primers spanning cdhR and PGN_1371 (left side) or PGN_1372 (right side). The presence of an amplicon with cDNA demonstrates cotranscription of PGN_1372 and cdhR. The No RT lanes are controls without reverse transcriptase. (C) Based on 5′ RACE, the transcriptional start site for cdhR and PGN_1372 was mapped to an A (bold and labeled +1) 22 bp upstream of the ATG start codon of PGN_1372. Primers used in the RT-PCR for (B) are underlined. Putative −10 and −35 promoter regions are labeled and underlined. Initiation codons are in bold italics, and termination codon is marked with a *.