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. 2001 Feb 27;98(5):2550–2554. doi: 10.1073/pnas.041611198

Figure 2.

Figure 2

Classical and nonclassical activators equally overcome telomeric repression, and that repression does not require Srb10. Yeast strains harboring a URA3-based reporter gene located at a telomeric (A) or internal (B) chromosomal locus were assayed for URA3 expression in the context of coexpressed classical (Gal4) and nonclassical (Gal11) activator molecules. Serial dilution spot assays on cell viability were performed by using plates with (+FOA) or without (−FOA) FOA. Decreased viability on +FOA plates indicates URA3 gene expression, whereas growth indicates URA3 repression by telomere position effect. (A) Promoter bound Gal4 and Gal11 can overcome telomeric repression equally well in both wild-type and ΔSrb10 or ΔSrb11 cells. The URA3 reporter contains binding sites for Gal4. Activators were tethered to the promoter via fusions to the DBD of Gal4 (residues 1–100). (B) When placed at an internal chromosomal locus, the URA3 reporter gene is expressed at sufficient levels even in the absence of an activator to render cells sensitive to FOA.