Table 1.
Repression of classical and nonclassical activation is Srb10/Srb11 dependent
| LexA-Gal4
|
LexA-Gal11
|
|||||
|---|---|---|---|---|---|---|
| DBD | DBD-Tup1 | Fold repression | DBD | DBD-Tup1 | Fold repression | |
| Wild type | 2,200 | 750 | 3.0 | 2,400 | 150 | 16.0 |
| ΔSrb10 | 720 | 710 | 1.0 | 1,190 | 1,010 | 1.2 |
| ΔSrb11 | 890 | 850 | 1.0 | 1,250 | 1,100 | 1.1 |
| Srb10-3 | 230 | 220 | 1.0 | 250 | 240 | 1.0 |
Repression of LexA-Gal4 and LexA-Gal11 was assayed as in Fig. 1 using wild type, Srb10 deleted (ΔSrb10), Srb11 deleted (ΔSrb11), and Srb10-3 (an SRB10 point mutant) strains carrying the reporter gene shown in Fig. 1. DBD represents the DNA binding domain of Gal4, residues 1–100. DBD-Tup1 represents Gal4 residues 1–100 fused to the N-terminal region of Tup1 residues 1–713. The numbers represent the average of assays on three independent colonies performed in duplicate. The standard errors were typically 15–20%.