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. Author manuscript; available in PMC: 2011 Jan 7.
Published in final edited form as: Neuron. 2005 Sep 15;47(6):833–843. doi: 10.1016/j.neuron.2005.08.022

Figure 5. T141K Substitutions Require a Negative Charge in the Channel Cavity.

Figure 5

(A–D) T141K substitutions require a negative charge in the cavity. Models are based on the KirBac1.1 structure with the position of T141K and the M2 acidic residue shown in CPK. I-V data and positions of the charged residues are shown for (A) T141K/S165D/D172N, (B) T141K/C169D/D172N, (C) T141K/D172, and (D) T141K/D172N/I176D. Similar results were obtained for the equivalent T141R mutants.

(E) Comparison of relative barium dissociation constants for channels with neutral cavities (D172N and T141M/D172N) and T141K, T141R, and T141M channels relative to wild-type at −80 mV.