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. Author manuscript; available in PMC: 2011 Jan 7.
Published in final edited form as: J Biol Chem. 2006 Mar 13;281(19):13169–13179. doi: 10.1074/jbc.M601010200

FIGURE 4. Mutation of D-sites of MKK7.

FIGURE 4

A, D1, D2, and D3 were mutated as shown, either alone or in combination, in the context of GST-MKK7-(1–85). The mutations are underlined. B, quantification of binding of MKK7 D-site mutants (40 µg) to JNK1, normalized to the binding of wild-type GST-MKK7-(1–85). Standard error bars are shown (n = 5). The key shows whether the indicated D-sites were wild-type (WT) (+), mutated (m), or absent (−). Shown below the graph are representative data bands showing the amount of 35S-JNK1 co-sedimented with the indicated GST-MKK7 mutant in a typical experiment.