TABLE 1. Oligonucleotides used in this study.

Where appropriate, restriction sites used for subcloning are underlined, and the translation initiation codon is shown in boldface.

Name	Sequence (5′ → 3′)	Use
JB812B	GCGGGATCCACCATGGCGGCGTCCTCCCTG	pGEX-MKK7 (1–38, 1–60, 1–85), pGEM-MKK7
JB818	CCGCTCGACCTACCTGAAGAAGGGGCGGTG	pGEM-MKK7
MKK7-(1–38)-R	GGCGTCGACTCACCGCTGGGGGCTGATATCCAG	pGEX-MKK7-(1–38)
MKK7-(1–60)-R	GGCGTCGACGCTCTCTGAGGATGGCGAGCGG	pGEX-MKK7-(1–60)
MKK7-(1–85)-R	GGCGTCGACGCGGGGTGTGAACAGGGTTG	pGEX-MKK7-(1–85)
M7For-(35–49)	GATCCAGCCCACAGCGGCCCAGGCCCACCCTGCAGCTCCCGCTGGCCAACG	pGEX-MKK7-D2
MKK7Rev-(35–49)	TCGACGTTGGCCAGCGGGAGCTGCAGGGTGGGCCTGGGCCGCTGTGGGCTG	pGEX-MKK7-D2
MKK7For-(67–80)	GATCCCTCCAGCTCGACCTCGACACATGCTGGGACTCCCTTCAACCTGAG	pGEX-MKK7-D3
MKK7Rev-(67–80)	TCGACTCAGGTTGAAGGGAGTCCCAGCATCTGTCGAGGTCGAGCTGGAGGG	pGEX-MKK7-D3
MKK4For-(36–49)	GATCCAGCATGCAGGGTAAACGCAAAGCACTGAAGTTGAATTTTGCAG	pGEX-MKK4-Ds
MKK4Rev-(36–49)	TCGACTGCAAAATTCAACTTCAGTGCTTTGCGTTTACCCTGCATGCTG	pGEX-MKK4-Ds
MKK7D1mutFor	GAGAACCGGGAGGCCGAGGAGGAGATCGACGCCAACGCGGATATCAGCCCGCAGCGGCCCAGG	pGEX-MKK7 D1 mutants, pcDNA-MKK7-D12-FLAG
MKK7D1mutRev	CCTGGGCCGCTCGGGCTGATATCCGCGTTGGCGTCGATCTCCTCCTCGGCCTCCCGGTTCTC	pGEX-MKK7 D1 mutants, pcDNA-MKK7-D12-FLAG
MKK7D2mutFor	CCTCAACCTGGATATCAGCCCGCAGGAGCCCGAGCCCACCGCGCAGGCCCGGCTGGCCAACGATGGG	pGEX-MKK7 D2 mutants pcDNA-MKK7-D12-FLAG
MKK7D2mutRev	CCCATCGTTGGCCAGCCGGGCCTGCGCGGTGGGCTCGGGCTCCTGCGGGCTGATATCCAGGTTGAGG	pGEX-MKK7 D2 mutants pcDNA-MKK7-D12-FLAG
MKK7D3mutFor	CCCGCAGCACCCGACGCCACCCGCCGAGCCCGAACACATGGCGGGCCTCCCGTCAACCCTGTTCACAC	pGEX-MKK7D3 mutants
MKK7D3mutRev	GTGTGAACAGGGTTGACGGGAGGCCCGCCATGTGTTCGGGCTCGGCGGGTGGCGTCGGGTGCTGCGGG	pGEX-MKK7D3 mutants
MKK7D2mutFixF	GCCAACGCGGATATCAGCCCGCAGGAGCCCGAGCCCACCGCGCAGGCC	pGEX-MKK7 D12 mutants pcDNA-MKK7-D12-FLAG
MKK7D2mutFixR	GGCCTGCGCGGTGGGCTCGGGCTCCTGCGGGCTGATATCCGCGTTGGC	pGEX-MKK7 D12 mutants pcDNA-MKK7-D12-FLAG
MKK7KDFor2	CAGGCCACATCATTGCTGTTGCGCAAATGCGGCGCTCTGGGAAC	pcDNA-MKK7-KD-FLAG
MKK7KDRev2	GTTCCCAGAGCGCCGCATTTGCGCAACAGCAATGATGTGGCCTG	pcDNA-MKK7-KD-FLAG