TABLE 5.
Selectivity ratios
| Selectivity of D-sites for MAPKs | |||
|---|---|---|---|
| D-site peptide | Noncognate/cognate | JNK substrate | Selectivity ratioa |
| MKK7-D1 | ERK2/JNK1 | c-Jun | 5.9 |
| MKK7-D1 | ERK2/JNK1 | ATF2 | 3.5 |
| MKK7-D1 | ERK2/JNK2 | c-Jun | 0.9 |
| MKK7-D1 | ERK2/JNK2 | ATF2 | <0.9 |
| MKK7-D2 | ERK2/JNK1 | c-Jun | 14.2 |
| MKK7-D2 | ERK2/JNK1 | ATF2 | 10.6 |
| MKK7-D2 | ERK2/JNK2 | c-Jun | 2.8 |
| MKK7-D2 | ERK2/JNK2 | ATF2 | 1.3 |
| MKK7-D3 | ERK2/JNK1 | c-Jun | 8.3 |
| MKK7-D3 | ERK2/JNK1 | ATF2 | 4.2 |
| MKK7-D3 | ERK2/JNK2 | c-Jun | 1.9 |
| MKK7-D3 | ERK2/JNK2 | ATF2 | 1.3 |
| MKK4 | ERK2/JNK1 | c-Jun | >10.0 |
| MKK4 | ERK2/JNK1 | ATF2 | 10.0 |
| MKK4 | ERK2/JNK2 | c-Jun | 6.7 |
| MKK4 | ERK2/JNK2 | ATF2 | 3.3 |
| MEK2 | JNK1/ERK2 | c-Jun | 2.7 |
| MEK2 | JNK1/ERK2 | ATF2 | 8.0 |
| MEK2 | JNK2/ERK2 | c-Jun | 14.0 |
| MEK2 | JNK2/ERK2 | ATF2 | >20.0 |
a
Selectivity ratio = IC50 (D-site peptide with noncognate MAPK)/IC50 (D-site peptide with cognate MAPK).