Table 1.
Identification and quantification of aconitase by dMS and SC.
dMS | Stage II vs. early Stage II | Stage III vs. Stage II | ||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
HarvEST Citrus ID | iCitrus ID | Blast Hit to TAIR ID | Ratio | Pvalue | No. peptides | Max. Xcorr | Ratio | Pvalue | No. peptides | Max. Xcorr | ||||||
UC46_7402 | 39802 | AT2G05710 | 24.9 | 9.9E-20 | 3 | 5.39 | 33.6 | 3.621E-07 | 4 | 4.56 | ||||||
UC46_5405 | 43680 | AT2G05710 | 25.3 | 9.9E-20 | 2 | 5.39 | 13.6 | 9.9E-20 | 2 | 4.56 | ||||||
UC46_5554 | 47264 | AT2G05710 | 62.0 | 9.9E-20 | 2 | 5.40 | 4.9 | 0.0007414 | 3 | 6.52 | ||||||
UC46_5555 | 45840 | AT2G05710 | 49.7 | 9.9E-20 | 4 | 5.40 | 4.8 | 0.0014824 | 4 | 6.52 | ||||||
UC46_9228 | 55395 | AT2G05710 | 25.3 | 9.9E-20 | 2 | 5.39 | 9.4 | 4.115E-06 | 3 | 5.07 | ||||||
Spectral Counting | Stage II vs. early Stage II | Stage III vs. Stage II | ||||||||||||||
HarvEST Citrus ID | iCitrus ID | Blast Hit to TAIR ID | Bayes Factor |
Fold Change |
Direction | No. peptides | -Log(e) | FDR Down | FDR up |
Bayes Factor | Fold Change |
Direction | No. peptides | -Log(e) | FDR Down | FDR up |
UC46_7402 | 39802 | AT2G05710 | 0.66 | 1.88 | 0 | 4 | 9.3 | 1.26 | 0.91 | 30.46 | 5.52 | 1 | 3 | 4.6 | 0.80 | 0.00 |
UC46_5405 | 43680 | AT2G05710 | - | - | - | - | - | - | - | 143.42 | 12.24 | 1 | 2 | 3.7 | 1.03 | 0.88 |
UC46_5555 | 45840 | AT2G05710 | 0.4 | 1.08 | 0 | 3 | 14.9 | 1.20 | 0.91 | 0.58 | 1.29 | 0 | 4 | 13.0 | 0.89 | 0.75 |
UC46_9228 | 55395 | AT2G05710 | 0.42 | 1.08 | 0 | 2 | 11.7 | 1.26 | 0.91 | 0.68 | 1.11 | 0 | 3 | 11.0 | 0.73 | 0.86 |
HarvEST | UC46_7402 | UC46_5405 | UC46_5555 | UC46_5554 | UC46_9228 | |||||||||||
Origin | Citrus ID | iCitrus ID | 39802 | 43680 | 45840 | 47264 | 55395 | |||||||||
C. Sinensis | UC46_7402 | 39802 | 87 | 17 | 18 | 86 | ||||||||||
C. Sinensis | UC46_5405 | 43680 | 87 | 86 | 86 | |||||||||||
C. Sinensis | UC46_5555 | 45840 | 99 | 90 | ||||||||||||
P. Trifoliata | UC46_5554 | 47264 | 89 | |||||||||||||
C. Sinensis | UC46_9228 | 55395 |
All iCitrus accessions for aconitase that were identified by both methods were homolog to the Arabidopsis gene At2g05710. Identification of aconitase by dMS and SC. The column "direction" under SC represents up-regulated = 1, no change = 0, down-regulated = -1. Aconitase iCitrus accessions amino acids sequences similarities.