Table 1. Crystallographic parameters for CusC.
| Data Collection | |
| Wavelength (Å) | 1.10 (NSLS beamline X6A) |
| Space group | R32 |
| Unit cell dimensions a,b,c (Å) | 89.03, 89.03, 473.04 |
| α,β,γ(°) | 90, 90, 120 |
| Resolution (Å) | 50−2.2 (2.24−2.20)1 |
| Rsym (%) | 13.0 (78.0) |
| Completeness (%) | 99.9 (99.6) |
| I/σ | 17.3 (2.8) |
| Redundancy | 7.4 (7.3) |
| Mosaicity (°) | 0.29 |
| Refinement | |
| Resolution range (Å) | 20−2.3 |
| Total no. of reflections | 31287 |
| Total no. of atoms in refinement | |
| Protein | 3349 |
| Sulphate | 35 |
| Water | 200 |
| Average B-factors (Å2) | |
| Protein | 33 |
| Sulphate | 81 |
| Water | 38 |
| Rwork/Rfree 2(%) | 19.8/24.3 |
| R.m.s. deviations from ideal | |
| Bond lengths (Å) | 0.009 |
| Bond angles (°) | 1.024 |
| Ramachandran plot statistics (%) | |
| Favored/allowed/disallowed | 93.6/5.9/0.53 |
1
Values in parentheses are for the highest resolution shell.
2
Rwork = Σ|Fo−Fc|/ΣFo. Rfree is the cross-validation of R-factor, with 5% of the total reflections omitted in model refinement.
3
The outliers of the Ramachandran plot (Asn100, Ser311) are located in the tips of the extracellular loops, which are poorly ordered.