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. 2011 Jan 7;6(1):e15935. doi: 10.1371/journal.pone.0015935

Figure 3. Real-time PCR.

Figure 3

Total inguinal and popliteal lymph node cells were obtained after immunization with pMAC/PS-scFv. The mRNA was extracted, and the expression of the scFv gene was measured by real-time PCR using a specific primer for the VH counterpart of scFv. The fold change was calculated based on GAPDH mRNA. Controls without reverse transcriptase were used for each pair. *p<0.05 compared with the controls (cardiotoxin or cardiotoxin plus empty pMAC). Results are representative of 3 independent experiments in which 8 mice in each group were used.