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. 2010 Aug 30;39(1):59–75. doi: 10.1093/nar/gkq741

Figure 3.

Figure 3.

TFIID-dependency of ectopic initiation induced by the TATA insertion was altered by the position where it was inserted within the RPS5 promoter. (A). TFIID-dependency of ectopic initiation sites induced by insertion of the TATA element at various positions as depicted in Figure 2A in the RPS5 promoter. Total RNA (20 µg) was isolated from strains carrying a combination of either TAF1 (left panel) or taf1-N568Δ (right panel) and either WT, #48, #49, #50, #51, #52, #53, #54, #55, #56, or #57 before, or 2 h after, a temperature shift from 30 to 37°C as indicated at the top. Primer extension analysis was done as described in Figure 2B. (B) Transcriptional initiation activities from the sites at −160, −133, −88, −71, −37, −27 and −23 bp in a taf1-N568Δ strain (right panel in A) are summarized schematically as in Figure 2D. Note that the regions conferring TFIID-independent or TFIID-dependent ectopic initiation sites when the TATA element was inserted are indicated at the bottom.