Table 1.
Gene fusions to selected global regulatory circuits
| Strain | Regulatory circuit | Fusion to: | Basal RLU* | Inducer | Response ratio† |
|---|---|---|---|---|---|
| DPD2247 | SOS | dinF | 0.224 | Nalidixic acid | 2.2 |
| DPD2248 | SOS | dinG | 32.8 | Nalidixic acid | 2.0 |
| DPD2249 | SOS | dinP | 4.41 | Nalidixic acid | 5.0 |
| DPD2250 | SOS | recA | 97.5 | Nalidixic acid | 1.8 |
| DPD2251 | SOS | recA | 99.5 | Nalidixic acid | 2.8 |
| DPD2253 | SOS | uvrA | ND‡ | Nalidixic acid | 1.4 |
| DPD2254 | SOS | uvrD | 15.7 | Nalidixic acid | 1.2 |
| DPD2293 | SOS | ruvA | 14.3 | Nalidixic acid | 4.6 |
| DPD2242 | Heat shock | lon | 6.71 | Ethanol | 5.6 |
| DPD2246 | Heat shock | rpoD | 9.49 | Ethanol | 2.4 |
| DPD2245 | Heat shock | rpoD | 8.07 | Ethanol | 6.8 |
| DPD2243 | Heat shock | clpB | 38.5 | Ethanol | 4.7 |
| DPD2244 | Heat shock | rfaDFCL | 33.1 | Ethanol | 1.4 |
| DPD2272 | SoxR/S | zwf | 16.9 | Methyl viologen | 3.6 |
| DPD2286 | SoxR/S | ribA | 5.8 | Methyl viologen | 4.7 |
| DPD2087 | SoxR/S | inaA | 9.8 | Methyl viologen | 9.0 |
| DPD3509 | SoxR/S | poxB | 1.5 | Methyl viologen | 15.0 |
| DPD2283 | OxyR | ahpC | 92.2 | Hydrogen peroxide | 16.5§ |
| DPD2284 | OxyR | ahpC | 68.3 | Hydrogen peroxide | 15.3§ |
*
From 100 μl actively growing cultures of E. coli DPD1675 transformants in minimal medium (15).
†
For nalidixic acid the response ratio was calculated at 110 min after addition; for ethanol, at 40 min; for methyl viologen, at 120 min; and for hydrogen peroxide, at 30 min.
‡
Not determined.
§
Response ratios from transformants of E. coli strain DPD2228.