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. 2010 Sep 3;39(1):347–358. doi: 10.1093/nar/gkq749

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© The Author(s) 2010. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 4. — NMR spectroscopy analysis of NT-SMG-9^12–180. (A) ¹H monodimensional spectrum of NT-SMG-9^12–180 showing the overlapping of signals in a narrow chemical shift in a range centered at 8.25 ppm. (B) ¹H-¹⁵N HSQC spectrum of ¹⁵N-labelled NT-SMG-9^12–180 unambiguously identified this domain as inherently unstructured due to the absence of well dispersed cross peaks. The presence of well-resolved peaks and the absence of dispersion discarded any non-specific aggregation.