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Several SMG-9-containing complexes can be isolated. (A and B) Size exclusion chromatography of SMG-1, SMG-8 and SMG-9 containing complexes. Fractions were run in SDS gels and the presence of either protein tested by western blot using anti-bodies specific to each component. As molecular weight markers, commercial markers (location of their elution peaks indicated in top line), mTOR (290 kDa and 0.7 MDa) and aPKCλ (78 kDa) were used.
