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MEF mRNA level is upregulated in p53−/− mice tissues. (A–F) Total RNA isolated from the indicated tissues of p53+/+ and p53−/− mice was analyzed for the expression of MEF by real-time quantitative RT–PCR. MEF mRNA level was normalized to mouse 18S rRNA (internal control). Results represent mean ± SD (n = 3). *P < 0.05, **P < 0.01 against p53 wild-type mice assessed by Student’s t-test.
