Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2010 Sep 14;39(1):381–392. doi: 10.1093/nar/gkq787

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2010. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

Figure 3. — Characteristics of active off-target sites. (A) The distribution of the number of matches to the target site for active (red) and inactive (blue) off-target sites with 5- or 6-bp spacing is shown. (B) Base frequency at each position in the ZFPL and ZFPR binding sites are displayed as a logo for the group of 18-active off-target sites. Guanines at seven positions (red boxes) in the binding sites were absolutely conserved within these sequences, while they are more variable in the inactive sites (Supplementary Figure S5). (C) The distribution of the number of active (red) and inactive (blue) off-target sites as a function of the number of guanines preserved in each recognition sequence. (D) Each base in the binding site of the ZFPL and ZFPR was independently mutated to cytosine, which is not found at any position in either of the ZFP recognition sequences, and its influence on ZFP binding was assayed using B1H-based activity assay (38) at 1 mM 3-AT. This assay will detect only the most important positions for recognition, where a reduction in cell survival (plotted as the –log of surviving colonies) indicates a position important for recognition. All of the absolutely conserved guanines—indicated by an asterisk—are critical for activity.