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. 2010 Oct 11;39(1):e2. doi: 10.1093/nar/gkq899

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© The Author(s) 2010. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 2. — Comparison of a 87-bp PCR amplification approaches for (A) a low-abundance (3%) T_m-increasing mutation (PFSK-1 cell-line DNA, c.823T > G; p.Cys275Gly), (B) a low-abundance (3%) T_m-equivalent mutation (HCC1008 cell-line DNA; c.841G > C, p.Asp281His), (C) a low-abundance (3%) T_m-reducing mutation (HCC2218 cell-line DNA; c.847C > T, p.Arg283Cys) and (D) a low-abundance (3%) T_m-reducing one base pair deletion (c.845del, p.Arg282del) in DNA from a clinical lung adenocarcinoma specimen. Representative chromatograms are presented; estimates of fold improvement are based upon multiple evaluations.