. 2010 Oct 11;39(1):e2. doi: 10.1093/nar/gkq899

Table 1.

Oligonucleotide sequences for ice-COLD-PCR; sequence orientation is presented 5′–3′

Oligo	Target	Sequence (5′–3′)
RS1 (RS60)
Ex8-167F^a	167-bp amplicon	GCTTCTCTTTTCCTATCCTG
Ex8-167R^a	167-bp amplicon	CTTACCTCGCTTAGTGCT
p53-87-F2^a	87-bp amplicon	TGGTAATCTACTGGGACG
p53-87-R^a	87-bp amplicon	CGGAGATTCTCTTCCTCT
30T-p53-87F	sequencing primer	TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTAATCTACTGGGACG
60refseq-for	RS60 target region	GGACGGAACAGCTTT
60refseq-rev	RS60 target region	CTGGCCGCGTGTCTC
RS60	60 nt WT RS	CTCTGTGCGCCGGTCTCTCCCAGGACAGGCACAAACACGCACCT CAAAGCTGTTCCGTCC-phosphate
Nested-87F	Nested-pyroseq	TGGTAATCTACTGGGACGGAA
Biotin-nested-87R	Biotinylated -pyroseq	Biotin-CGGAGATTCTCTTCCTCTGTG
RS-Pyro-Seq-primer	Sequencing primer	TGCCTGTCCTGGGAG
RS2 (RS90)
Ex8-167F^a	167-bp amplicon	GCTTCTCTTTTCCTATCCTG
Ex8-167R^a	167-bp amplicon	CTTACCTCGCTTAGTGCT
p53-ex8-115F	115-bp amplicon	TTGCTTCTCTTTTCCTAT
p53-ex8-115R	115-bp amplicon	GCGGAGATTCTCTTC
40T-p53-115F	sequencing primer	TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCTTCTCTTTTCCTATCC
90refseq-for	RS90 target region	CTTCCTCTGTGCGCC
90refseq-rev	RS90 target region	CTATCCTGAGTAGTG
RS90	90 nt WT RS	CTTCCTCTGTGCGCCGGTCTCTCCCAGGACAGGCACAAACACGCACCTCAAAG CTGTTCCGTCCCAGTAGATTACCACTACTCAGGATAG-phosphate

^aPrimers previously reported in ref. (9).