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. Author manuscript; available in PMC: 2012 Jan 14.
Published in final edited form as: J Mol Biol. 2010 Nov 12;405(2):548–559. doi: 10.1016/j.jmb.2010.11.012

Figure 1. Reconstitution of the Mtw1 complex.

Figure 1

A. Expression and purification of the Mtw1 complex. The coomassie-stained gel shows different steps of the purification scheme: control extract and extract after induction with IPTG, eluate from Ni-NTA beads and purified fraction after SEC. Note the presence of degradation products of the Dsn1p subunit. B. Expression and purification of a complex with an N-terminally truncated Dsn1p subunit. C. Expression and purification of a stable Dsn1-Nsl1 heterodimer. IEX denotes anion exchange chromatorgraphy. Asterisks in A, B and C indicate a contamination with the E. coli Hsp70 chaperone D. Expression and purification of Mtw1-Nnf1 heterodimer. E. SEC runs of Dsn1p-Nsl1p and Mtw1p-Nnf1p subcomplexes (8 μM each) and of the full complex after reconstitution. F. Coomassie-stained SDS-PAGE of fractions from E. Asterisks indicate an Nnf1 truncation product.