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. Author manuscript; available in PMC: 2012 Feb 5.
Published in final edited form as: Virology. 2010 Dec 4;410(1):216–227. doi: 10.1016/j.virol.2010.11.012

Figure 2. The RRV DC5692 strain and virus derived from a molecular clone of the DC5692 strain (RR87) are attenuated in vivo.

Figure 2

(A) Twenty-four-day-old C57BL/6J mice were inoculated with PBS (n = 3), or 103 PFU of RR64 (n = 4), RRV strain DC5692 (n = 5), or the molecular clone of DC5692, RR87 (n = 5) by injection in the left rear footpad. Mice were scored for the development of disease signs including loss of gripping ability, hind-limb weakness, and altered gait. Each data point represents the arithmetic mean ± standard deviation (SD). Data was evaluated for statistically significant differences by ANOVA (P < 0.0001) followed by Bonferroni’s Multiple Comparison Test. (B) At 10 dpi, mice were sacrificed and perfused with 4% paraformaldehyde. 5 micron-thick paraffin-embedded sections generated from quadriceps muscles were stained with H&E. Images are representative of three mice per group.